Task N.7: site-directed mutagenesis


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overlap extention

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Modified: 6 October 2009, 9:12 PM   User: Perroteau Isabelle  → 

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PCR-mediated overlap extension can create specific nucleotide mutations or generate chimeric gene products.


Figure a illustrates....



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Figure b illustrates.......



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From the following article: Gene splicing and mutagenesis by PCR-driven overlap extension Karin L Heckman & Larry R Pease Nature Protocols 2, 924 - 932 (2007)