This teaching contributes to the learning objectives included into the Biomedical and Biomolecular area of the Master in Cellular and Molecular Biology, providing knowledge and applicative abilities to critically investigate cell functions. Students learn to address issues related to the complex relationship between structural and functional components of the cell. Starting from the basic role of diffusional events that will be treated from both the theoretical and experimental points of view, particular attention will be focused on live cell imaging techniques as experimental tools to investigate physiological processes in time and space at different biological scales (from molecular to multicellular). For each item, students learn how to highlight the specific scientific question, to interpret data, to discuss the experimental approaches employed by the authors. The main objective is to develop the ability to critically analyze and interpret the results of the related scientific literature.