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LM: Biologia Cellulare e Molecolare Avanzata (2009-2010)
Assignment BMG6 - what can we conclude ?

Opened: Thursday, 26 November 2009, 3:45 PM
Due: Sunday, 10 January 2010, 3:45 PM

To deal with this assignment, you should read results from the article of Kim et al., here attached.

In Figure 4, panels B and C, results are shown of genomic mapping realized with chromatin immunoprecipitation, relative to AcH3 (acetyl-lysine in Histone H3) and to MeH3K4 (methyl-lysine 4 in Histone H3), which are specific modifications found in nucleosomes in proximity of promoters.

Looking at results obtained for the known gene PIK4CB (panel B) and for the unknown locus ENm010 (panel C),what can be conclude about the role of these histone modifications ?

1. In which part of the T.U. are modifications clustered ?

2. What you can guess about the roles of these histone modification on the transcription of genes?

◄ Article: Identification of human promoters (Kim et al 2005)
Assignment BMG7: micro RNA specificity ►
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