elongation
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| PCR-mediated overlap extension can prodice specific nucleotide mutations or generate chimeric gene products. Figure a illustrates the PCR-mediated overlap extension methods leading to specific mutations. Four primers are used that have the following characteristics.... Figure b illustrates the PCR-mediated overlap extension methods leading to chimeric gene products. Four primers are used that have the following characteristics.... |
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| From the following article Gene splicing and mutagenesis by PCR-driven overlap extension Karin L Heckman & Larry R Pease Nature Protocols 2, 924 - 932 (2007) | |
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