Task N.7: site-directed mutagenesis
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Transposons
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Modified: 9 October 2009, 7:17 PM User: Teixeira Maira →
MsoNormal">Transposons are mobile genetic elements that can move from one site to another in the genome. This transposition can cause mutations and change the amount of DNA in the genome. Transposons were also once called "jumping genes", and are examples of mobile genetic elements. Class I mobile genetic elements, or retrotransposons, copy themselves by first being transcribed to RNA, then reverse transcribed back to DNA by reverse transcriptase, and then being inserted at another position in the genome. Class II mobile genetic elements move directly from one position to another using a transposase to "cut and paste" them within the genome. Although the procedure for in vitro mutagenesis using transposons is widely used, mutations in DNA are not site specific, as in other techniches.