Task N.7: site-directed mutagenesis


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elongation

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Modified: 6 October 2009, 8:58 PM   User: Perroteau Isabelle  → 

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PCR-mediated overlap extension can prodice specific nucleotide mutations or generate chimeric gene products.

Figure a illustrates the PCR-mediated overlap extension methods leading to specific mutations. Four primers are used that have the following characteristics....




Figure b illustrates the PCR-mediated overlap extension methods leading to chimeric gene products. Four primers are used that have the following characteristics....
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From the following article Gene splicing and mutagenesis by PCR-driven overlap extension Karin L Heckman & Larry R Pease Nature Protocols 2, 924 - 932 (2007)